Lipbarvis® unveils the skin barrier

Lipbarvis® takes a non-invasive look into the skin

Microscopy Services uses the patented Lipbarvis® (Lipid Barrier Visualization, LBV) analysis method to examine the epidermal skin barrier directly without invasive sampling.

We take skin samples in the range of seven to eight cell layers using a particularly gentle adhesive/carrier system. These are then available for various investigations.

With our innovative analysis method, you receive clear and reliable statements about your development processes. Facts rather than estimates - with Lipbarvis® without negative environmental effects, without a large pool of test persons.

Lipbarvis® provides the answers to your dermatological questions

The investigation potential of this analysis method is acutely adapted to your questions about the lipid barrier: If you are planning studies on atopic dermatitis, dry skin and psoriasis, or if you need meaningful analyses and image material on the condition of the lipid barrier or other target structures in the stratum corneum before and after the application of your care product or pharmaceutical agent - Lipbarvis® reliably provides you with the urgently needed answers.

Lipbarvis® is the only recognized method that directly illustrates the condition of the skin barrier and the effect of cosmetics or pharmaceuticals.

Lipbarvis® is considerably more sensitive than conventional biophysical methods and provides clear proof of efficacy when, for example, cornometry or TEWL have long since reached their analytical limits. In addition, morphological results can be reliably correlated with the determination of skin lipids, skin hydration and epidermal proteins. Lipbarvis® is even suitable for lipid determinations (Lipbarvis® LIP, LBV-LIP). Further statements and recommendations are thus possible and support the arguments benefitting your product marketing.

Lipbarvis® had breadth and depth:

with before/after comparison of lipid lamellae (LBV-TEM), with determination of skin lipids (LBV-LIP), with analysis of cell volumes (LBV-SEM)

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Current publications with Lipbarvis

R.Fölster-Holst, C.Naß, S.Dähnhardt-Pfeiffer, S.Freitag-Wolf (2022) "Analysis of the structure and function of the epidermal barrier in patients with ichthyoses—clinical and electron microscopical investigations"  J Our Academy Dermatologie Venerol 2022 May;36(5):726-738.

D.Ditgen, D.Segger, J.Klaucke, D.Dähnhardt, S.Dähnhardt-Pfeiffer, D.Westphal, J.Degwert (2022) "Effects of cigarette smoke on the skin in comparison to UV radiation: parallels and differences" 3-2022 EURO COSMETICS 26-32

R.Fölster-Holst, R.Reimer, C.Neumann, E.Proksch, E.Rodriguez, S.Weidinger, M.Goldust, E.Hanisch, S.Dähnhardt-Pfeiffer, S.Freitag-Wolf (2021) "Comparison of Epidermal Barrier Integrity in Adults with Classic Atopic Dermatitis, Atopic Prurigo and Non-Atopic Prurigo Nodularis" Biology 2021, 10, 1008.

F. Hüppop, S. Dähnhardt-Pfeiffer, R.Fölster-Holst (2021) "Characterization of Classical Flexural and Nummular Forms of Atopic Dermatitis in Childhood with Regard to Anamnestic, Clinical and Epidermal Barrier Aspects" Acta Derm Venereol 2022; 102: adv00664

D.Dähnhardt, M.Bastian, S.Dähnhardt-Pfeiffer, M.Buchner, R.Fölster-Holst (2020) “Comparing the effects of proactive treatment with tacrolimus ointment and mometasone furoate on the epidermal barrier structure and ceramide levels of patients with atopic dermatitis” J.Dermatological Treatment,

D.Dähnhardt, S.Dähnhardt-Pfeiffer, Bundesverband Handschutz e.V. (2019) „ Lipbarvis im beruflichen Hautschutz: Innovative Methode zur Untersuchung der epidermalen Hautbarriere nach Anwendung von Waschlösungen“. Dermatologie in Beruf und Umwelt Jahrgang 67 Nr.3 p.124 Download Poster

A. Kilic, C.Masur, H.Reich U.Knie, D.Dähnhardt, S.Dähnhardt-Pfeiffer, C.Abels (2019) "Skin acidification with a water-in-oil emulsion (pH4) restores disrutes epidermal barriers and improves structure of lipid lamellae in the elderly". Journal of Dermatology 2019; oo; 1-9 doi: 10.1111/1346-8138.14891

D.Dähnhardt, C.Surber, S.Dähnhardt-Pfeiffer (2018) "Influence of Topical Formulations: Lipid Lamella Organization and Lipid Composition of Stratum Corneum as a Surrogate Marker for Barrier Integrity. Surber C, Abels C, Maibach H (eds): pH of the skin: Issues and Challenges. Curr Probl Dermatol. Basel, Karger, 2018 vol 54, pp 166-172 (DOI:10.1159/000489530)

C.Masur, U.Knie, A.Kilic, D.Dähnhardt, S.Dähnhardt-Pfeiffer, C.Abels (2018) "Imporoved Structure of lipid lamellae and restoration of disrupted epidemal barrier in the elderly after four week treatment with a water-in-oil emulsion with pH 4.  Posterpresentation : Perspectives in Percutaneous Penetration Conference, La Grand Motte, April 2018 Download Poster

A.Oláh, J.Szabó-Papp, M.Soeberdt, U.Knie, S.Dähnhardt-Pfeiffer, C.Abels, T.Biró (2017) "Echinacea purpurea-derived alkramides exhibit potent anti-inflammatory effects and alleviate clinical symptoms of atopic eczema" . J. Dermatol Sci. doi: 10.1016/j.jdermsci.2017.05.015

H.Stettler, P.Kurka, N.Lunau, C.Manger, A.Böhling, S.Bielfeldt, K.-P. Wilhelm, S.Dähnhardt-Pfeiffer, D.Dähnhardt, F.H.H.Brill, H.Lenz (2016) "A new topical pantheon-containing emollients: Results from two randomized controlled studies assessing its skin moisturization and barrier restoration potential, and the effect on skin microflora", J. of Dermatological

J.Blaak, D.Dähnhardt, S.Dähnhardt-Pfeiffer, S.Bielfeld, K.-P. Wilhelm, R.Wohlfart, P.Staib (2016) "A Plant oil-containing pH4 emulsion improves epidermal barrier structure and enhances ceramic levels in aged skin".  International J. of Cosmetic Science 2016 1-8, doi:10.1111/ics.12374

D.Dähnhardt, S.Dähnhardt-Pfeiffer, J.Schulte-Walter, T.Neubourg, E.Hanisch, C.Schmetz, M.Breuer, R.Fölster-Holst (2016) "The Influence of Two Different Foam Creams on Skin Barrier Repair of Foot Xerosis: A Prospective Double-Blind, Randomised,Placebo-Controlled, Intra-Individual Study"  Skin Pharmacol Physiol, (2016) 29:266-272

E.Proksch, D.Dähnhardt, S.Dähnhardt-Pfeiffer, R.Fölster-Holst (2016) "Epidermale Barrierestörung bei Dermatosen".Hautarzt (2016) DOI 10.1007/s00105-016-3883-2

S. Dähnhardt-Pfeiffer, D. Dähnhardt, C. Schmetz, M. Buchner, R. Fölster-Holst (2014) „Corneocyte membrane undulation and intercellular lipid membranes as an indicator for severity in netherton syndrome, ichthyosis vulgaris and atopic dermatitis? Comparison of SC ultrastructures and barrier lipids in a pilot study using electron microscopy and Lipbarvis®.“ JDDG (2014) 12s2: 1610-0379/

J.-M. Jensen, M. Weppner, S. Dähnhardt-Pfeiffer, C. P. Neumann, M. Bräutigam, T. K. Schwarz, R. Fölster-Holst, Proksch, E. (2013) „Effects Of Pimecrolimus Compared With Triamcinolone Acetonide Cream On Skin Barrier Structure In Atopic Dermatitis: A Randomized, Double-Blind, Right-Left Arm Trial.“ Acta Derm Venereol (2013) 93: 515–519

S. Dähnhardt-Pfeiffer, D. Dähnhardt, M. Buchner, K. Walter, E. Proksch, R. Fölster-Holst (2013)„Comparison of effects of tacrolimus ointment and mometasone furoate cream on the epidermal barrier of patients with atopic dermatitis.“ JDDG (2013) 4: 437-443

R. Fölster-Holst, S. Dähnhardt-Pfeiffer, D. Dähnhardt, Proksch E. (2012) „The role of skin barrier function in atopic dermatitis: an update.“ Expert Review of Dermatology (2012) 7/3:247-257

D. Dähnhardt, C. Surber, K.-P. Wilhelm, G. Springmann, R. Fölster-Holst, S. Dähnhardt-Pfeiffer (2012) „Direct Evaluation of the Barrier Integrity by Visualization of the Intercellular Lipid Lamellae using TEM. Pilot Study with an Emollient on Children with Atopic Skin.“ 16. DG Jahrestagung 1-3. März 2012, Berlin

S. Daehnhardt-Pfeiffer, C. Surber, K.-P. Wilhelm, D. Daehnhardt, G. Springmann, M. Boettcher, R. Foelster-Holst (2012) „Noninvasive Stratum Corneum Sampling and Electron Microscopical Examination of Skin Barrier Integrity: Pilot Study with a Topical Glycerin Formulation for Atopic Dermatitis.“ Skin Pharmacol Physiol (2012) 25:155-161